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In negative ionization mode, PC [M+Ac]− and PS [M−H]− species are isobaric, as shown in the table within the figure. Unknown lipid signals (gray circles) from 6–7 min in the HILIC chromatogram were plotted onto trendlines fit to CCS values of known PS (long dashed line) and PC (short dashed line) species. The unknown lipids were determined to be PCs because their CCS values fall onto the PC trendline.
