Fig. 10.

Effect of the cho1Δ mutation on the level of Pah1 during cell growth. Wild-type and the cho1Δ cells were grown in synthetic complete medium supplemented with 1 mM choline. The cells at the indicated time of growth were lysed and subjected to immunoblot analysis with anti-Pah1 antibody. The amounts of Pah1 on the immunoblot were determined by fluoroimaging. The amount of Pah1 from the wild-type or cho1Δ mutant was normalized to the amount of that found at the 16 h time point. The lack of the Cho1 protein in the cho1Δ mutant was confirmed by immunoblot analysis with anti-Cho1 antibody. The yeast cells, grown for 16–20 h, were in the exponential phase, whereas those grown for 36 h were in the stationary phase. The data shown are the averages of three experiments ± SD (error bars).