Figure 6.

Tubulin inhibitor 1 and caged derivative 2 combined with UV irradiation induce apoptosis in RN1 glioblastoma cells. Cells were treated with 1 and 2 (5 μM ± UV irradiation) for 48 h. Control cells received an equivalent amount of DMSO or UV irradiation (365 nm, 1800 mW, 30 s). Cells were stained with Annexin V and analyzed using the MUSE Cell Analyzer. Data represent mean ± SEM from three independent experiments (****P < 0.0001, one-way ANOVA followed by Tukey’s multiple comparison test).