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. 2017 Apr 17;18:17. doi: 10.1186/s12860-017-0134-z

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Expression of PUMA upon overexpression of the MDM2 splice variants. Activation of PUMA after transfection with the splice variants by qPCR assay revealing relative mRNA levels of PUMA. MCF-7 cells transfected with pCMV-GFP (GFP-control), MDM2-A, -B and -C, sorted after 12 h based on GFP expression. Graphs show the untreated cells (purple bars) and the 24 h 1 μM doxorubicin (green bars). The experiment was repeated in triplicate, with three independent transfections. * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001