Figure 4.

SDS extract of 20,000 microdissected alveolar septum cells separated by SDS-PAGE. After first extraction with UTC buffer a second extraction step with SDS sample buffer was performed. The amount equivalent to 20,000 cells of this extract was separated on a 15% SDS gel. Band 1 and band 2 correspond to the selected protein peaks of 13.8 and 14.0 kD from WCX Array experiments (Figure 2).