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. 2017 Mar 15;31(6):617–627. doi: 10.1101/gad.292409.116

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© 2017 Ueda et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — HDG11 binds to the WOX8 cisC fragment and is expressed in the female gametophyte, the zygote, and the embryo. (A) Yeast one-hybrid assay of interactions between HDG11 and the indicated WOX8 cis-regulatory fragments. (B) Two-photon microscope images showing pHDG11:YFP expression in ovules containing a mature embryo sac with the egg cell (female gametophyte) or the zygote. Autofluorescence is shown in magenta. Arrowheads point to the nucleus of the egg cell (ec), synergid cell (sy), central cell (cc), and zygote (zyg). (C) pHDG11:YFP expression during embryogenesis at the indicated stages. (Inset) Enhanced YFP signal without differential interference contrast image. Bars, 10 µm.