Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Mar 1;31(5):524–535. doi: 10.1101/gad.294546.116

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Isomura et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Figure 1. — Optogenetic perturbation system. (A) Schematic of the light-inducible gene expression system based on hGAVPO. Blue-light illumination activates hGAVPO, which induces downstream gene expression under the control of the UAS promoter. Here, the effects of 3′ untranslated region (UTR) sequences on the optogenetic input were examined by using the ubiquitinated nuclear localization signal (Ub-NLS)-luciferase (Luc) construct. (B) The effects of 3′ UTR sequences on light-induced gene expression patterns. (c.p.s.) Counts per second. (C) The effects of 3′ UTR sequences on light-induced gene expression patterns in B were normalized. (D–F) Single-cell bioluminescence imaging of oscillatory gene expression patterns induced by the construct containing the Hes1 3′ UTR with a 3-h period of blue-light illumination. (D) Snapshots for a single cell imaged for 13.5 h. (E) A heat map of single-cell traces. Each line represents a cell. (F) Population (red) and single-cell (gray) time series.