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. 2017 Feb 16;26(8):1552–1564. doi: 10.1093/hmg/ddx059

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© The Author 2017. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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In situ expression of miR-210 positively correlates to hypoxia marker CAIX in CRC tissue. (A–C) Scatter plots of miR-210, miR-21 and miR-30d expression (1/2^−ΔCT) assessed by qRT-PCR and percentage of CAIX-positive staining within the tumour (n = 13). Correlation efficiency calculated with Spearman’s Rank analysis showed a significant positive correlation between miR-210 and CAIX expression CRC tumours (D and E) IHC of CAIX and in situ hybridization of miR-210 performed on serial sections of FFPE blocks of CRC tumours (n = 8). Staining of miR-210 was observed in the epithelial cells along the crypts and overlapped with CAIX staining within these cells.