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. 2017 Feb 16;26(8):1552–1564. doi: 10.1093/hmg/ddx059

Figure 6.

Figure 6

MiR-30d modulates 5-FU resistance in hypoxic DLD-1 cells. (A) Venn diagram of common miRNAs that are dysregulated in both arrays (in vivo hypoxia array and ex vivo CRC tissue array). (B) DLD-1 cells were transfected with miRNA mimic and inhibitors under normoxic and hypoxic conditions. Experimental timeline of the experiments is shown. (C) Cell survival following treatment with 2 mM 5-FU and vehicle (DMSO). Data normalized to untreated cells. (D and E) Graph represents fold change in survival following treatment with 5-FU and vehicle. Data normalized to vehicle and then to its transfection control (NTC) inhibition of miR-30d under hypoxic conditions resulted in a significant reduction in resistance to 5-FU. Bars represent mean values with SEM calculated from three individual experiments. *P < 0.05, **P < 0.01.