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. 2017 May;23(5):735–748. doi: 10.1261/rna.060541.116

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© 2017 Celik et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — Validation of several different classes of NMD substrates by Northern blotting. Northern blotting analyses of (A) intron-containing transcripts (HRB1, RPL22B, NHP6B, and MTR2), (B) transcripts using frameshifting during translation (Ty-4 transposons), (C) “noncoding” RNAs (ICR1 and IRT1), and (D) negative control transcripts (HAC1 pre-mRNA). Total RNA was isolated from the indicated strains, and the steady-state levels of individual transcripts in these strains were analyzed by Northern blotting. In each case, a random-primed probe was hybridized to the blot and SCR1 served as the loading control.