FIGURE 2.

Observation of stall-induced cleavage requires active translation and the absence of Dom34. (A) Average ribosome occupancy for short reads (top dark traces) and long reads (bottom light traces) from genes with UAA stop codons and no in-frame stop codons in the 3′UTR. Gene sequences are aligned at their stop codons and reads are mapped by their 5′ ends. ORF annotation drawn to coincide with ribosome A sites. (B) Average ribosome occupancy from genes with UAA stop codons and no in-frame stop codons in the 3′UTR. Gene sequences are aligned at their primary polyadenylation sites and reads are mapped by their 5′ ends and unshifted. Only genes translating 3′UTRs after nonsense suppression generate mRNA cleavage and resultant short-read enrichment in the dom34Δ strain.