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. 2017 May;23(5):749–761. doi: 10.1261/rna.060418.116

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© 2017 Guydosh and Green; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 4. — Ribosome profiling data reveals minor pausing on polylysine sequences but no mRNA cleavage upstream. Average fraction of ribosome occupancy from the dom34Δ* strain over a window around one or more consecutive Lys codons found within open reading frames. Only the first instance of multiple repeats was included when multiple repeats were consecutively encoded. Data are shifted so that the ribosome P site corresponds to the first codon position (0–2 nt) on the plot (16 nt shift). The appearance of accumulating ribosomes (pausing) when Lys codons are positioned in the E, P, and A sites of the ribosome is indicated with a red arrow for the KKK motif. Replicate data sets are from a previous study (Guydosh and Green 2014). Yellow line indicates continuing pausing when the lysines move into the exit tunnel. Green underline indicates region of artifactual short read enrichment downstream from pause region in only one replicate.