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. 2017 Mar 27;114(15):3909–3914. doi: 10.1073/pnas.1614664114

Fig. 1.

Fig. 1.

Common transcriptional targets of ERRα and LSD1. (A) Heatmap of the 985 genes over- or underexpressed upon both siERRαs or both siLSD1s using log twofold changes (scale indicated). (B) Venn diagrams schematizing the number of genes modulated (up or down, Top) or activated (Bottom) by ERRα (pink) and/or LSD1 (blue). See Dataset S1 for complete gene lists. (C) Expression of the indicated genes analyzed by RT-qPCR after transfection with the indicated siRNA, relative to control conditions. Values are mean ± SEM of three independent experiments performed in triplicate. (D) ChIP experiments using anti-ERRα (Upper) and anti-LSD1 (Lower) antibody or IgG. Percent enrichments relative to input were measured by qPCR, amplifying a region encompassing the TSS for LSD1 or putative ERREs for ERRα. Bars represent mean ± SEM of three independent experiments performed in duplicate. Significance is shown relative to control conditions. *P < 0.05; **P < 0.01; ***P < 0.005; ns, nonsignificant.