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. 2017 Mar 27;114(15):3909–3914. doi: 10.1073/pnas.1614664114

Fig. S3.

Fig. S3.

ERRα transcriptional activity depends on LSD1 and controls for ChIP assays. (A) Cells inactivated for LSD1 (siLSD1#1 and #2) or not (siC) were transfected with ERRE-luciferase plasmid together with increasing amounts of ERRα-encoding plasmid. Results were normalized to samples transfected by ERRE-luciferase only. Bars show the mean ± SEM of three independent experiments performed in triplicate. (B) Detection of H3K4me2 and H3K9me2 at the TSSs of the indicated genes analyzed by ChIP. IgG was used as a control. Enrichments are presented relative to total H3 with bars representing mean ± SEM of three independent experiments performed in duplicate. (C) Detection of H3K9me2 at the ERREs of the indicated gene after siRNA-mediated inactivation of ERRα or LSD1. Results are expressed relative to control conditions with bars showing the mean of two independent experiments performed in triplicate. (D) Same as Fig. 2, analyzing the TSSs of the indicated genes. Significance is indicated relative to control conditions. *P < 0.05; ***P < 0.005; ns, nonsignificant.