Fig. 3.

The early β-catenin signature contains previously undescribed dorsal transcripts. (A–I) Box plots indicate dorsal and ventral gene expression levels (in RPKM) in five independent regenerating half-embryo experiments. These genes have either not been previously characterized or have been only superficially investigated. Box plots illustrate the reproducibility of replicate experiments. Each dot represents the expression in RPKM of the corresponding gene in a distinct experiment. Genes above the whisker are considered outliers according to the interquartile range (IQR) calculated for each box plot. The difference between dorsal and ventral expression was statistically significant for all nine genes, with all genes surpassing the 95% confidence interval mark at a minimum. The β-CatMO RPKMs (β) were similar to those of ventral halves. Statistical significance was determined through two-tailed pairwise P values. Significant differences in expression values (RPKM) are indicated as *≤0.05, **≤0.01, and ***≤0.005. (J) In situ hybridization validating that unknown Loc100170590 is indeed expressed in Spemann organizer mesoderm at gastrula. White arrowhead indicates the dorsal blastopore lip. (Magnification: 16×.) (K) At tailbud stage 24, Loc1001170590 is expressed in eye, central nervous system, somites, and proctodeum. (Magnification: 14×.) D, dorsal; St., stage; V, ventral.