Fig. S5.

Functional characterization of the constructs used in this study (related to Fig. 4). (A) Amino acid sequence alignments for Ciona GluA, rat GluA1, and rat GluA 2, around the Q/R editing site (arrow). (B) Representative current recordings in response to glutamate application in the presence or absence of Ca²⁺. Bars at the top indicate the timing of application of glutamate and Ca²⁺. Current amplitudes were measured at the points before glutamate application (black arrows), at the current peak in 0 mM Ca²⁺ (blue arrows), and in 5 mM Ca²⁺ (red arrows). (C) The Ca²⁺ permeability index was calculated by normalizing [current increase (at +60 mV in 5 mM Ca²⁺)] by [current increase (at –120 mV in 0 mM Ca²⁺)]. This is to normalize for the GluA expression level. N.S., not significant; r, rat.