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. 2017 Mar 27;114(15):3999–4004. doi: 10.1073/pnas.1616874114

Fig. 4.

Fig. 4.

BRF110 neuroprotection against 6-OHDA and AAV-ASYN toxicity in mice. (A) Decreased number of apomorphine-induced contralateral turns in C57BL/6 mice injected unilaterally with 6-OHDA and treated daily with either vehicle or BRF110 for 13 d, showing greater than eightfold improvement. n = 5. (B) Rotarod test of mice subjected to unilateral injection of 6-OHDA and receiving either vehicle or BRF110, showing 12-fold improvement with BRF110 treatment. n = 5. (C) SN TH IHC images of mice receiving unilateral injections of 6-OHDA and treated with either vehicle or BRF110. (D) Stereological counting of SN TH(+) neurons in mice receiving unilateral injections of 6-OHDA and treated daily with either vehicle or BRF110, which increased the number of TH(+) neurons by 47%. n = 5. (E and F) Striatum TH IHC of mice receiving 6-OHDA injections and treatment with either vehicle or BRF110, showing 10-fold increased innervation. n = 5. (G) Stereological counting of SN TH(+) neurons of mice receiving unilateral injections of AAV-ASYN and treated daily with either vehicle or BRF110, which increased the number of TH(+) neurons by 48%. n = 7. (H and I) SN TH IHC images of mice receiving unilateral injections of AAV-ASYN and contralateral injections of AAV-GFP and treated with either vehicle or BRF110. (J and K) Striatum TH IHC of mice receiving injections of AAV-ASYN or AAV-GFP and treated with either vehicle or BRF110, showing 10-fold increased innervation (L). n = 7.