Fig. S3.

HPLC analysis of BRF110 at 214 nm (98% purity) (A), 254 nm (95.8% purity) (B), and 280 nm (95.3% purity) (C). HPLC analysis of BRF110 was performed on an Agilent Eclipse XDB-C18 column with particle size of 5 μm (15 cm × 4.6 mm i.d.), using an Agilent 1200 series liquid chromatograph equipped with a 20-μL sample loop injector. The peaks representing the target analyte(s) were recognized by both their retention time and their spectrum pattern recorded on a diode array detector running Agilent ChemStation chromatography software. Elution was accomplished isocratically with a solvent system consisting of 75% CH₃CN-25% H₂O (0.1% TFA). HPLC chromatograms of BRF110 were recorded in three different wavelengths to assess purity.