Characterization of iPSC-derived human DAergic neurons. Human DAergic neurons were differentiated from neural progenitor cells for 2 wk. (A) Expression of relevant markers detected by qPCR for MAP2, TH, FOXA2, and LMX1 (n = 3). (B) Differentiation into midbrain-specific DAergic neurons, shown by immunohistochemistry staining for MAP2, TH, and LMX1. (Scale bars: 50 µm.)