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. 2017 Mar 27;114(15):3957–3962. doi: 10.1073/pnas.1619863114

Fig. 5.

Fig. 5.

Radio-resistant cells require RelB to support normal hematopoiesis. (A) Flow cytometry analysis of live B220− splenocytes from chimeras generated with CD45.2+ WT or Relb−/− BM into CD45.1+ B6.SJL recipients or B6.SJL BM into Relb−/− mice, analyzed 8 wk after lethal irradiation and transplantation. Data are representative of two independent experiments. (B) Percentage of cDCs (CD11c+MHC-II+) in the indicated BM chimeras generated as in A. Each dot represents a biological replicate from two independent experiments. (C) Flow cytometry analysis of live granulocytes in spleens of BM chimeras generated as in A. (D) Frequency of Ly-6G+ PMNs in spleens of the indicated chimeras. Each dot represents a biological replicate from two independent experiments. (E) Splenocyte counts in the indicated BM chimeras 8 wk after irradiation and reconstitution. (F) Thymic DCs in the indicated mice were analyzed by flow cytometry. BM chimeras were generated as in A. Shown are two-color histograms representative of thymocyte samples pregated as CD45.2+B220−. (G) Frequency of DCs within CD45.2+B220− thymocytes in the indicated mice. (H) Frequency of thymic DC subsets in the indicated mice. Each dot represents an individual biological replicate from two independent experiments. ****P < 0.0001.