Fig. 2.
Etv5 is required to maintain AT2 cell fate in vitro. (A, Left) Colonies formed by AT2 cells after culture on Matrigel with or without growth factors. (Right) Each symbol indicates cells from one mouse. Data are shown as mean ± SEM. **P < 0.01, *P < 0.05 (Student's t test). (B, Left) Heatmap shows expression of AT1 and AT2 marker genes (7) in cells sorted and then cultured with 4-OHT. RNA-seq values are scaled and centered, normalized counts to account for library size and gene length (n = 4 mice per genotype). (Right) The volcano plot indicates the effect of Etv5 deficiency on AT1 (blue) and AT2 (red) marker genes. (C) Immunostaining of colonies formed by AT2 cells after induction of Krasd12.