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. 2017 Mar 28;114(15):3903–3908. doi: 10.1073/pnas.1621177114

Fig. 6.

Fig. 6.

Active ERK stabilizes ETV5 by inhibiting the COP1 ubiquitin ligase. (A) Organization of the CRL4COP1/DET1 ubiquitin ligase. NED, Nedd8. (B) Immunoblots of E1A/Hras-transformed MEFs treated with MEK inhibitor (MEKI) for 1 h. (C) Immunoblots of H460, H1299, and Krasd12ΔTrp53 (KP1 and KP2) cells transfected with Cop1 siRNAs and then treated with ERK inhibitor (ERKI) for 1 h. SiNC1, siRNA negative control 1. (D) Immunoblots of human NSCLC lines treated with MLN4924 for 1 h before the addition of ERK inhibitor.