Fig. 1.

FAK Y397F mutation is early embryonic lethal. (A) Bright-field images of E9.5 and E10.5 wild-type (WT) and Y397F embryos. DA, dorsal aorta; ISV, intersomitic vessel. (B) Whole-mount pictures of E9.5 WT and Y397F embryos stained for CD31 (purple). (C) Higher magnification of CD31-stained vasculature. Arrowheads point to a web of small vessels in WT embryos. (D) E10.5 control and Y397F yolk sacs. (E) Immunohistochemistry staining of E9.5 embryo frozen sections using the indicated antibodies. CA, cardiac mesoderm. (F) SPP1 RNA copy numbers as determined by quantitative PCR in E9.5 whole-embryo–derived RNA. *P = 0.002, Student’s t test. n.s., not significant. (Scale bars, 200 μm.)