Fig. 2.
Experimental work flow. (A) Male gptΔ B6C3F1 mice were treated as neonates with AFB1 and killed at either 10 wk (A-10) or 72 wk (A-72) of age. D-10 and D-72 are the corresponding DMSO solvent controls. (B) Liver tissues from killed mice at 72 wk of age were subjected to collagenase perfusion, and hepatocytes or tumor cells were isolated (A-72H and A-72T, respectively; D-72 is the corresponding DMSO control). (C) Overview of the DS method used to identify mutational spectra obtained from the mice shown in A and B. Adapted from Schmitt et al. (24). Details are provided in the main text. CAT, chloramphenicol acetyl transferase.