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Sequences of the adapter and PCR oligonucleotides
| Name | Sequence |
| Top adapter | 5′-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′ |
| Bottom adapter | 5′Phosphorylation-TCTTCTACAGTCANNNNNNNNAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG-3′ |
| PCR primer 1 | 5′-AATGATACGGCGACCACCGAGATCTACACAGAGAGACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′ |
| PCR primer 2 | 5′-CAAGCAGAAGACGGCATACGAGATXXXXXXCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-3′ |
Where XXXXXX in primer 2 indicates the position of fixed multiplexing barcode sequence.
