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Depletion of Fkh1-mCherry. GAL1pr-FKH1-mCherry fkh2∆ cells were grown as described in Methods on galactose containing media overnight (GAL1pr on). Cells were then diluted in either YP + 1% galactose 1% raffinose or YP + 2% (wt/vol) glucose (GAL1pr off) media to OD 0.05 as for Hi-C experiments. Cells were harvested and run on Western blot. For Hi-C experiments, cells were treated as with the glucose treatment and harvested at OD 1, at which point Fkh1 was undetectable.
