Fig. S4.

cPALM is compatible with cryoEM. Cryogenic electron micrographs of frozen-hydrated tobacco mosaic virus (TMV), a well-characterized test specimen, and projections of their Fourier transform intensities parallel to the equator. (A) “Untreated” TMV specimen that was only washed in liquid ethane at −145 °C. (B) “Treated” TMV specimen that was dipped in 4-methyl-1-pentene at −145 °C and then washed in liquid ethane at −145 °C. Both treated and untreated samples gave rise to sixth-order layer lines corresponding to a resolution of at least 1.2-nm resolution. This level of resolution is what one expects for the transforms of single particles. The exposure to the cryofluid and its removal were successful with no detectable damage to the TMV.