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. 2009 Feb;150(2):580–591. doi: 10.1210/en.2008-0726

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Fig. 4. — Contribution of IGF-I receptor activation to GLP-1 secretion. A, Semiquantitative RT-PCR was performed on isolated RNA from GLUTag (lane 2) and NCI-H716 (lane 5) cells for IGF-I receptor mRNA transcripts. Total RNA from mouse fat (lane 3) or human jejunal tissue (lane 6) was used as a positive control, and the omission of template RNA was used as a negative control (lanes 1 and 4). B and C, The effect of IGF-I receptor activation on GLP-1 release was analyzed by treating GLUTag (n = 6/group) (B) and NCI-H716 (n = 4–6/group) (C) cells with graded concentrations of human Long-Arg3-IGF-I for 2 h. GLP-1 secretion was determined by RIA, and all data are expressed as a percent of the untreated control. *, P < 0.05.