Fig. 3.

Hepatic Foxo inactivation increases blood triglyceride and cholesterol levels and promotes lipid secretion. Concentrations of serum triglyceride (A) and total cholesterol (B) in 18-h fasted mice were measured. *, P < 0.05 (n = 6). C, The content of fatty acids in seven different lipid classes in serum from 18-h fasted control (CNTR) and F1/3KO mice were measured. Data were the average ± sem from three different experiments in which samples from each group were from a pool of serum from five mice. CE, Cholesterol ester; FA, free fatty acids; TAG, triglycerides; LYPC, lysophosphatidylcholine; PC, phosphatidylcholine; PE, phosphatidylethanolamine; FC, free cholesterol. D, Serum total cholesterol, HDL-cholesterol, and LDL/VLDL-cholesterol levels were measured in 18-h fasted control, F1KO, F3KO, and F1/3KO mice using a commercial assay kit. Error bars represent sem. *, P < 0.05 vs. control mice (n = 4). E, Liver triglyceride secretion was analyzed in 12-wk-old control, F1KO, F3KO, and F1/3KO mice that were fasted for 5 h and then injected with tyloxapol by tail vein. Serum triglyceride concentrations were measured at 30, 60, 90, and 120 min after tyloxapol injection. Error bars represent sem. *, P < 0.05 vs. control mice (n = 4). F, Lipoprotein ApoB-100 levels in serum were analyzed by immunoblotting in 5-h fasted mice or mice after 2 h of tyloxapol injection (20 μg protein/each lane) from control, F1KO, F3KO, and F1/3KO mice. Data are representative from at least three different experiments. *, P < 0.05 vs. control (n = 3).