Table 2.
Hydrogen peroxide elimination ([H2O2]=5 μM) and toxicity. Cell cultures: embryonic stem cells (ESCs), their differentiated progeny cells (difESCs) and endometrial mesenchymal stem cells (eMSCs).
Cells |
|||
---|---|---|---|
Parameter | ESCs | difESCs | eMSCs |
kcell, s−1 cell−1 L | 3.0±0.5×10–12(N=5) | 16±2×10–12 (N=4) | 10±1×10–12 (N=4) |
kprot, s−1 g−1 L | 3.7±0.6×10−2 (N=5) | 4.7±0.5×10−2 (N=4) | 2.0±0.2×10−2 (N=4) |
LD50cell, pmole/cell | 1.0±0.3 (N=3) | 11±1 (N=2) | |
LD50prot, mmole/protein g | 12±4 (N=3) | 20±2 (N=2) |
Notations: kcell and kprot, first-order rate constants for the external H2O2 removal quantified on the per-cell, or per-protein basis, correspondingly; LD50cell and LD50prot, cytotoxic thresholds assessed as H2O2 dose which resulted in 50% cell death and estimated either in mole-per-cell, or mole-per-protein units, correspondingly.