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. 2015 Jan 16;156(4):1540–1551. doi: 10.1210/en.2014-1371

Figure 5. NIS expression in FRTL-5 cells remained unchanged after acute excess I−.

Figure 5.

A, Representative picture of immunoblots of NIS expression in FRTL-5 cells treated with 100 μM I− for 2 hours in the presence or absence of ROSsc. E-cadherin was probed as a loading control in the same nitrocellulose. B, The graph depicts densitometry analysis of the 86-kDa band of NIS normalized with respect to the value obtained from the densitometry analysis of the E-cadherin band. The data were plotted as the mean ± SEM (n = 3). The value corresponding to 100% was the control group. C, Representative immunoblot of a cell surface biotinylation experiment analyzing NIS content at the plasma membrane in FRTL-5 cells incubated with excess I− in the presence or absence of ROSsc. D, The graph depicts the NIS content quantified by a densitometry analysis of the 85-kDa bands. Results were expressed as the mean ± SEM (n = 3), where the control group was set at 100%.