FIG. 2.

Tyrosine hydroxylase immunocytochemistry of rat brain removed 72 h after a single intravenous injection of 10 μg per rat of clone 951 plasmid DNA encapsulated in PIL targeted with either the TfRmAb (panels A–C) or with the mouse IgG2a isotype control (panels D–F). Coronal sections are shown for three different rats from each of the two treatment groups. Clone 951 is the TH expression plasmid under the influence of the human GFAP promoter. The 6-hydroxydopamine was injected in the medial forebrain bundle of the right hemisphere, which corresponds to right side of the figure. Sections are not counterstained. Reproduced with permission from Zhang et al. Normalization of striatal tyrosine hydroxylase and reversal of motor impairment in experimental parkonsinism with intravenous nonviral gene therapy and a brain-specific promotor. Hum Gene Ther 15:339–350. Copyright © 2004, Mary Ann Liebert, Inc. All rights reserved.²⁵