Figure 7.

DNA Methylation of Fbp2 after Exposure to 25 mM High Glucose (>8 wk) and after Normalization (5.6 mM) for 1 Week. Location of (A) Fbp2 relative to (B) amplicon regions assayed and (C) CpG sites using UCSC Genome Browser (http:/genome.ucsc.edu/). Black bars indicate individual CpG sites, red bars indicate decreased methylation following high glucose, and green bars indicate increased methylation. E–J, Percent methylation was obtained for each CpG assayed, location corresponding to CpG ID (D), (n = 8), mean ± SEM. one-way ANOVA followed by Dunnett's Multiple Comparison Test compared with 5.6 mM control; *, P < .05; **, P < .01; ***, P < .001.