Table 1.
Real-Time PCR Primer Sequences
| Target Gene | Forward Primer (5′→3′) | Reverse Primer (3′→5′) | Accession Number |
|---|---|---|---|
| POMC | CAAGAAGCGGCGCCCTGTGA | GCTGCTCGCCTTCCAGCTCC | NM_139326.2 |
| AgRP | AGAGTTCTCAGGTCTAAGTCT | TTGAAGAAGCGGCAGTAGCACGT | NM_033650.1 |
| RPLP0 | GCAGGTGTTTGACAACGGCAG | GATGATGGAGTGTGGCAC | NM_022402.2 |
| CPT1A | CGCTCATGGTCAACAGCAACTACT | CTCACGGTCTAATGTGCGACGA | NM_031559.2 |
| F4/80 | CCAGCTTATGCCACCTGCA | TCCAGGCCCTGGAACATTGG | NM_001007557.1 |
| CD11c | CCTGAGGGTGGGCTGGATGCCA | GCCAATTTCCTCCGGACA | NM_001037780.2 |
| TNF-α | GCTACGGGCTTGTCACTC | CCACGCTCTTCTGTCTACTG | NM_012675.3 |
| IL-6 | TCCTACCCCAACTTCCAATGCTCT | TTGGATGGTCTTGGTCCTTAGCC | NM_012589.2 |
| Leptin (Ob) | GACATTTCACACACGCAGTCG | GAGGAGGTCTCGCAGGTT | NM_013076.3 |
| Arginase-1 | GGCAATTGGAAGCATCTCTGGC | CTGTGATGTAGAGACCTTCTC | NM_017134.3 |
| Adiponectin (ACRP-30) | AATCCTGCCCAGTCATGAAG | TCTCCAGGAGTGCCATCTCT | NM_144744.3 |
| Rn18s | CGCGGTTCTATTTTGTTGGT | AGTCGGCATCGTTTATGGTC | NR_046237.1 |