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. 2005 Jan;2(1):139–150. doi: 10.1602/neurorx.2.1.139

FIG. 4.

FIG. 4.

Screening of shRNA constructs directed to the EGFR. Top: A series of shRNA constructs directed to the EGFR mRNA were prepared. Both nucleotide number and the relative position in the target EGFR mRNA are indicated in the figure. The U6 shRNA expression vectors were prepared as described in Figure 2 for luciferase shRNA plasmids. Bottom: The RNAi efficacy of anti-EGFR constructs was investigated in human U87 glioma cells incubated with [3H]-thymidine for a 48-h period that follows lipofection with the plasmid DNA of interest. The shRNA clone 967 and the control antisense-RNA plasmid clone 882 produced maximum inhibitory effect in the incorporation of thymidine in human U87 cells. Clone 882 is a 700-nt antisense RNA complementary to nt 2317-3006 of the human EGFR driven by the SV40 promoter and containing the EBNA-1/oriP elements.30 Data are mean ± SEM (n = 3 dishes). Reproduced with permission41 (see Table 1 legend).