Full line represents linear regression and dotted lines represent 95% prediction interval. A, 0pM–77pM; slope (95%CI) = 173 (163–184); y-intercept (95%CI) = −200 (−486–487). B, 0pM–7.7pM; slope (95%CI) = 108 (103–113), y-intercept (95%CI) = 60 (43–77). C, 0pM–1.5pM; slope (95%CI) = 104 (94–114), y-intercept (95%CI) = 61 (52–70).