Skip to main content
. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: J Surg Res. 2016 Oct 20;209:191–198. doi: 10.1016/j.jss.2016.10.014

Figure 1. BK-mediated PKD phosphorylation at Ser-916 is inhibited by CID755673.

Figure 1

Confluent 18Co cells were washed and equilibrated in serum-free media for 30 min, then exposed to BK (100nM) for 60 min in the presence or absence of the PKD inhibitor CID755673 (10 µM). Cell lysates were then analyzed by SDS-PAGE and Western blot using an antibody that detects phosphorylated PKD protein (pPKD) at serine residue 916 (Ser-916). BK induced the phosphorylation of PKD at Ser-916, an effect that significantly inhibited by CID755673 (CID) at 15, 30 and 60 min. The results shown are the mean ± S.E. n ≥ 3 and are expressed as a relative expression level of phosphorylated PKD, displayed in graphical form below. Equal protein loading was verified using an antibody that detects total PKD protein. * denotes p < 0.05.