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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: J Surg Res. 2016 Oct 20;209:191–198. doi: 10.1016/j.jss.2016.10.014

Figure 5. BK-induced myofibroblast migration is inhibited by Hsp27 siRNA and NS398.

Figure 5

Panel A: 18Co cells were transfected with 100 nM Hsp27 siRNA or with a non-targeting sequence as described in Materials and Methods. The surface of a confluent monolayer of transfected 18Co cells was scratched with a sterile pipette tip. Three pictures were randomly taken along the scratch gap and pictured positions were marked (time 0 h). Cells were then treated with BK (100nM) for 24 h. Three pictures were taken after 24 h at the same positions as marked previously. The scratch gap area was measured with software Image J and normalized to time 0 control. The results shown are the mean ± S.E. n ≥ 3 and are expressed as percentage decrease in scratch gap area. * denotes p < 0.05. Panel B: The surface of a confluent monolayer of 18Co cells was scratched with a sterile pipette tip and incubated in serum free medium. Three pictures were randomly taken along the scratch gap and pictured positions were marked (time 0 h). Cells were then treated with BK (100 nM) for 24 h, in the presence or absence of NS398 (10 µM), a COX-2-specific inhibitor. Three pictures were taken after 24 h at the same positions as marked previously. The scratch gap area was measured with software Image J and normalized to time 0 control. The results shown are the mean ± S.E. n ≥ 3 and are expressed as a percentage decrease in scratch area. * denotes p < 0.05.