Fig. 1.

Proteome characterization during HSV-1 infection by mass spectrometry. A, Overview of the HSV-1 lytic lifecycle (top) across the six time points of viral infection in HFF cells. For both host and virus we identified and quantified proteome, phosphoproteome and chromatin-associated proteins. The host proteome was also investigated for histone PTMs. B, Total number of quantified proteins for host (blue) and virus (orange) for each time point and biological replicate. C, Histogram representing the number of host proteins quantified across all conditions. Most proteins were detected and quantified at all six time points. Proteins quantified in at least 5 out of 6 conditions were analyzed using fuzzy c-means clustering and functional enrichment using Gene Ontology (GO), whereas the others were only subjected to GO analysis. D, Top 50 host proteins with the largest coefficient of variation across the six time points, i.e. the proteins with the largest changes in intensity during virus infection. Data are sorted by ratio early/late time points. E, Log₂ transformed absolute intensity (iBAQ) of all identified viral proteins. Data were clustered using a hierarchical tree (Euclidean).