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. 2017 Feb 8;16(4 Suppl 1):S92–S107. doi: 10.1074/mcp.M116.065987

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 6. — Validation of changes in histone marks during HSV-1 infection of HFF cells. A, Western blot analysis of selected histone marks quantified by MS over the time course (3–15 hpi) of HSV-1 infection in HFFs. The different marks are indicated and compared with uninfected mock (M) extracts. B, Ponceau stain of lysates used in (A) showing even loading of histone proteins in all samples. C, Immunofluorescence imaging of H3K9ac (green) at 3, 6, and 9 hpi. ICP8 (red) marks viral replication compartments and DNA is stained with DAPI (blue). Scale bar is 10 μm. D, Same as (C) for H3K14ac.