Fig. 2.

Protein levels of IFIX-GFP decrease by 4 h post-infection and remain diminished over the course of HSV-1 infection in primary fibroblasts. A, time course of EGFP or IFIX-GFP HFF cells during infection with BFP-HSV-1 analyzed by Western blotting. The blot reveals a decrease in IFIX-GFP levels over time that is not due to GFP being targeted or cleaved. B, late times of WT HSV-1 infection in IFIX-GFP stable HFFs analyzed by Western blotting showing the lack of IFIX-GFP signal late in infection. C, still images extracted from live cell microscopy movies, taken with ×60 objective, monitoring IFIX-GFP in uninfected (mock) and BFP-tagged HSV-1 infected stable HFF cells (see also supplemental Movie S1). Bar, 5 μm. D, schematic of the changes in IFIX sub-nuclear localization and levels during HSV-1 infection. Small green dots represent diffuse IFIX recruitment to distinct puncta that are dispersed over time.