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Sa grows to a higher density when grown in a nutrient rich environment. The optical density at 600 nm of four independent colonies of LAC WT grown in both minimal (RPMI) and rich (TSB) media was measured at t = 0, 2, 4, 6, and 8 h. Minimal medium (RPMI) colony density is plotted in red and rich medium (TSB) is plotted in blue. The density of the cultures after 2 h is slightly higher when grown in a nutrient rich environment.
