Fig. 3.

A CaaX motif is required for FCP localization. A, Parasites expressing Wt GFP-FCP or GFP-FCP(C322S) were labeled with AlkFOH and GFP-FCP was immunoprecipitated. AlkFOH labeling was visualized by a click reaction with AzTAMRA (top panel) which loading was visualized by an immunoblot against GFP (bottom panel). B, Live epifluorescent images of P. falciparum parasites expressing either Wt GFP-FCP (top panel) or GFP-FCP(C322S) (bottom panel). The nucleus was stained using Hoechst. Additional images are shown in the supplemental material (Supplemental Fig. S1).