Fig. 4.

Quantitative proteomic analysis of translation initiation during MNV infection. SILAC-based quantitative proteomics was employed to investigate changes to eIF composition during high MOI (10) MNV infection of BV-2 cells. The experimental layout is illustrated in (A) with samples taken at mock (0 h) early (4 h) or late (9 h) post infection either lysed or subject to m7GTP-Sepharose purification. A Coomassie gel (B) and representative Western blots (C) confirm initiation factor enrichment. Venn diagrams illustrating experimental proteome coverage in (D) whole-cell lysate, or E) m7GTP-Sepharose pulldowns.