Fig. 1.

Schematic overview of the workflow. A detailed overview of the proposed workflow, from sample preparation to data handling and data analysis is shown. A, The selection of the Baccus peptides used in the bacterial cell counting method. B, Sample preparation methods and data analysis for the absolute quantification of proteins. The colors of proteins in the sample preparation: blue, plasma proteins; green, intracellular bacterial proteins; red, M1-protein, the main S. pyogenes surface proteins. C, Outline of the steps used for determining of the stoichiometric density model from the absolutely quantified data (B) and final construction of the surface density model.