Fig. 3.

Significant differences in protein abundance levels in the SRM data. The absolute quantification of the plasma proteins binding to the surface of S. pyogenes, measured using SRM. A, Two methods were used to elute surface bound human plasma proteins from the SF370 strain; bacteria digest (gray bars) and glycine elution (white bars). B, Wild type strain SF370 (gray bars) and an M1-deletion mutant ΔM1 (white bars). Only human proteins with significant difference (p > 0.01) between the two elution methods are shown in (A) or the two strains shown in (B) are included in the figure. The y-axis displays the absolute quantified value of each protein in femtomole per injection into the MS. The error bars represent the standard deviation of the eight replicates.