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. 2017 Apr 15;26(8):608–616. doi: 10.1089/scd.2016.0289

FIG. 3.

FIG. 3.

(A) Comparison of osteogenic differentiation between nonheat shock and heat shocked ASCs cryopreserved 3 h postheat shock in 10% PVP. Left: Alizarin red S staining after 21 days of osteogenic differentiated samples and their controls. Right: quantification of eluted Alizarin red stain. (B) Comparison of adipogenic differentiation between nonheat shock and heat shocked ASCs cryopreserved 3 h postheat shock in 10% PVP. Left: Oil O red staining after 14 days of adipogenic differentiated samples and their controls. Right: quantification of eluted Oil O red stain. (C) qPCR osteogenesis—the expression of osteogenic genes Runx2, osteonectin, and osteopontin remained unaffected in the heat shock (HS) cryopreserved ASCs in comparison with cryopreserved ASCs. *indicates P value <0.05 as statistically significant. NS as not significant (P > 0.05). (D) qPCR adipogenesis—the expression of adipogenic genes PPARg and adiponectin remained unaffected in the heat shock (HS) cryopreserved ASCs in comparison with cryopreserved ASCs. The mRNA for adiponectin has higher expression level than the other two adipogenic genes. *indicates P value <0.05 as statistically significant. NS as not significant (P > 0.05). qPCR, quantitative polymerase chain reaction.