Table 1.
Yeast Strains
| Strain (Synonym) | Genotype | Autonomous plasmid | Reference (Source) |
|---|---|---|---|
| YA101 (MCY726) | MATaura3-52 his3-Δ200 ade2-101 lys2-801 | M. Carlson | |
| BY4743 | MATa/MATα ura3Δ/- leu2Δ/- his3Δ1/- LYS2/lys2Δ0 met15Δ0/MET15 | wiki.yeastgenome.org/index.php/ Commonly_used_strains | |
| YB225 | MATa-inc ura3 rad4::KanMX | This laboratory [42] | |
| YB226 | MATa-inc trp1 ura3 his3 ade2 rad4::KanMX rad51::URA3 | This laboratory [42] | |
| YB318 | MATα ura3-52 his3-Δ200 ade2-n trp1-Δ1 gal3−leu2-3, 112 GAL1::his3-Δ5' trp1::his3-Δ3'::HOcs | This Laboratory [34] | |
| YB400 | MATa-inc trp1 ura3 his3 ade2 rad4::KanMX rad51 | Derived from YB226 [42] | |
| YB406 | MATaura3-52 trp1::hOR his3-Δ 200 ade2-101 lys2-801 | This Laboratory [34] | |
| LSY1957 | MATaYFP-rad51-I345T-URA3-RAD51 ADE2 leu2 trp1 ura3 his3 | L.Symington | |
| YB407 | MATα ura3-52, trp1::hOR his3-Δ200,ade2-101 lys2-801 | This Laboratory | |
| YB419 | MATaYFP-rad51-I345T-URA3-RAD51 ADE2 leu2 trp1::hOR ura3 his3 | Meiotic segregant of diploid cross of LSY1957 and YB407 | |
| YB420 | MATaYFP-rad51-I345T-URA3-RAD51 ADE2 leu2 trp1::hOR ura3 his3 | pRS424 | Trp+ transformant of YB419 |
| YB425 | MATα ura3-52 ura3-52 his3-Δ200 ade2-n trp1-Δ1 gal3−leu2-3, 112 GAL1::his3-Δ5' trp1::his3-Δ3'::HOcs rad4::kanMX | Derived from cross of YB318 and YB225 | |
| YB426 | MATa ura3-52, trp1::hOR his3-Δ200,ade2-101 lys2-801 rad4 ::KanMX | Derived from cross of YB407 and YB225 | |
| YB427 | MATa/MATα ura3-52/- his3-Δ200/- ade2-101/ade2-n trp1-Δ1/trp1::hOR leu2-3,112/leu2-801 GAL1::his3-Δ5' trp1::his3-Δ3'::HOcs rad4::kanMX/rad4 kanMX | Diploid cross of YB425 and YB426 | |
| YB428 | MATa/MATα ura3-52/- his3-Δ200/- ade2-101/ade2-n trp1-Δ1/trp1::hOR leu2-3,112/leu2-801 GAL1::his3-Δ5' trp1::his3-Δ3'::HOcs rad4::kanMX/rad4 kanMX | pRS424 CYP1A1 | Trp+ transformant of YB427 |
| YB429 | MATa/MATα ura3-52/- his3-Δ200/- ade2-101/ade2-n trp1-Δ1/trp1::hOR leu2-3,112/leu2-801 GAL1::his3-Δ5' trp1::his3-Δ3'::HOcs rad4::kanMX/rad4 kanMX | pRS424 CYP1A1 I462V | Trp+ transformant of YB427 |
| YB430 | MATa/MATα ura3-52/- his3-Δ200/- ade2-101/ade2-n trp1-Δ1/trp1::hOR leu2-3,112/leu2-801 GAL1::his3-Δ5' trp1::his3-Δ3'::HOcs rad4::kanMX/rad4 kanMX | pRS424 CYP1A1 T461N | Trp+ transformant of YB427 |
| YB431 | MATa-inc ura3 rad4::KanMX rad51 | PRS424 CYP1A1 | Trp+ transformant of YB400 |
| YB432 | MATa-inc ura3 rad4::KanMX rad51 | pRS424 CYP1A1 I462V | Trp+ transformant of YB400 |
| YB433 | MATa-inc ura3 rad4::KanMX rad51 | pRS424 CYP1A1 T461 | Trp+ transformant of YB400 |
| YB434 | MATaYFP-rad51-I345T-URA3-RAD51 ADE2 leu2 trp1::hOR ura3 his3 | pRS424 CYP1A1 | Trp+ transformant of YB400 |
| YB435 | MATaYFP-rad51-I345T-URA3-RAD51 ADE2 leu2 trp1::hOR ura3 his3 | pRS424 CYP1A1 I462V | Trp+ transformant of YB419 |
| YB436 | MATaYFP-rad51-I345T-URA3-RAD51 ADE2 leu2 trp1::hOR ura3 his3 | pRS424 CYP1A1 T461N | Trp+ transformant of YB419 |