Figure 4.
Progestin (R5020) mediated increase in ψm is not dependent on protein synthesis. A, An overall significant treatment effect was seen (P < .001). Pairwise comparisons of a difference in ψm include the following: P < .001, R5020 vs EtOH; P < .001, R5020 vs CHX; and P = .385 CHX vs CHX + R5020. A significant difference was seen between day of treatment and ψm (P = .034). B, The efficacy of CHX was determined by the inhibition of the known induction of the DNA-binding protein inhibitor, inhibitor of DNA binding 1 (ID-1), by TGFβ. Reactions were performed in the medium or Krebs-Ringers-HEPES (KRH) plus glucose buffer. Western blot analyses of hTERT-HM cell protein after 60 minutes of treatment with 5 ng/mL TGFβ-1, with or without 2 hours of pretreatment with 5 μg/mL CHX, were conducted. All results are expressed as mean ± SEM. Treatments with different superscripts are significantly different. Exps, number of experiments done on different days; Obs, total number of observations in all experiments.