Figure 5.
Progestin (R5020) increases ψm in hTERT-HM cells expressing PR-M. A, RT-PCR showing high expression of rPR-M transcript in transfected hTERT-HM cells (TR) compared with untransfected (Ut) and control (Ctl) transfected hTERT-HM cells (top panel). There was no difference in GAPDH transcript levels (bottom panel). B, R5020 (10−7 M) increases ψm in hTERT-HM cells (black bars) expressing PR-M (rPR-M-R) compared with R5020-treated control (gray bars) transfected cells (Ctl-R) (P = .019). Results are from three assays with six to eight replicates per assay. Fluorescent units (x-axis) for TMRM emission were corrected for background. All results are expressed as mean ± SEM. rPR-M-V, recombinant PR-M-expressing cells treated with vehicle; rPR-M-R, recombinant PR-M-expressing cells treated with R5020; OMA, oligomycin; Ctl-V, control transfected cells treated with vehicle; Ctl-R, control-transfected cells treated with R5020.